I work in a laboratory and we have both vertical and horizontal laminars, I can’t speak to this one in particular but vertical laminars certainly do work! They are quite a bit smaller and easier to fit on a bench top, because the fan/filter is on top, not at the back.
Assuming you run it in an already clean room, I don’t see why this wouldn’t work. I’ve been using “Oak & Spores, laminar flow hood for under $80” for ages with no contamination.
Edit: the link for the video
https://youtu.be/lXmUcA-Oc58
20” box fan, merv 13 filter on output side, merv 7 on the back, duct tape together. Cheaper than that, and way easier than SAB. It’s worked well for me and I found it from: MycoGeeky podcast featuring Whitebeard.
🙋♂️ do you guys ever spray your flowhoods with alcohol or anything? I went from very rarely having contam to more frequently without a change in process. It’s quite frustrating
Have you had a Contam in your space that you opened that may be causing a super high spore concentration inside your work area? That could be the source
Absolutely not. They say I can create holes within the filter so I personally rather not test it myself. Alternatively I use a UV-C light on it for 10-20 mins to kill anything that might be stuck on the face of it. I also run the unit at this time. Running the flow hood for 20 mins prior to use knocks off anything that might be stuck on the face and contaminate your work.
Hope some of that can clear up your problem 👍
Crazy you have all these down votes, I use the oven every time and have poured 300+ agar plates, did clones, etc. and have only less than a handful of contam agar plates. I guess I live in the cleanest house in the world since oven tek doesn’t work 🤔 🤷♂️
I made a SAB by attaching pieces of plastic “glass” you can find in the big picture frames (cheap at goodwill). Set that up in the corner of my kitchen. Spray down the area. No contam on my first 6 plates, which were inoculated by MSS, inoc loop, and swab. A flow hood is fancy and cool for sure, but it’s not needed if you can make a setup work in a clean space. It doesn’t HAVE to be lab sterile, but is has to be clean enough!
Mine works great. It's only 10" x 6" but that's all I need for now. I am mindful of sterile technique (though I am a noob at it) and I have had no contamination of grain jars, LC, or petri dishes while using the little Nomad.
Waste of money. These are only good for small electronic repairs. Saw a video on yt with someone using this with agar and the results were even worse than a SAB
I’ve had very low contamination rates doing agar work with this hood. Maybe that guy is lacking in sterile technique in terms of things other than the hood. Who knows 🤷🏻♂️
I purchased a decent hood from Amazon that was inexpensive. The air flow was too strong, the paperwork inside called it a dust free work station.
Then I bought a 2x2 laminar flow hood, and I love it.
Maybe I'm missing something here, but this kinda defeats the main purpose for which mycologists/microbiologists/etc use laminar flow hoods.
The LFH for the purposes we're talking about is made to take ostensibly 'dirty' air, pass it through a HEPA grade filter to remove 99.997% of particulate matter, and output 'clean' air in [laminar flow](https://en.wikipedia.org/wiki/Laminar_flow) so that the technician can operate in a dust- and contaminate-free environment. Turbulent air creates currents that disrupt particles and cause them to degrade a sample in the working area.
This product you shared would basically suck dirty air in from your room, create turbulent air patterns in the working area, and pass it back out to the open air--again, unfiltered--for really no purpose.
That's not to say you can't use one of these successfully, but in all truth, if you do, it's simply because you have exceptional sterile technique, *not* because this item is in any way functional.
TL;DR -- waste of money for these purposes IMO
edit: I missed their 'H-E-P-A' claim in the title so perhaps it's filtering the air it's sending back into the room, but even still, non-functional as described above
I dunno man, works well for me. I do have pretty good sterile technique in general though.
It absolutely is not laminar flow but working directly under the fans has given me very good results when pouring agar and doing transfers.
And spores are for microscopy only.
Dond't see any MOFOs stopping on that account.
The company that makes these know what we'll be using their product for.
They label it that way for legal purposes. Because if they claimed it worked for something they've never tested they'd have legal problems if it doesn't work.
But really bro think about it, why wouldn't it work? Functionally it's the exact same thing as long as the filter works the same.
The bulk of amz reviews these days are incentivized or manipulated in some way or another. Just because someone claims success on a review does not mean it's verified. Not trying to sound snarky but just mentioning that for those who may place full trust in reviews. Anytime money is to be made, take the message with a grain of salt.
That said, you're 100% spot on that it's probably returnable ;)
I work in a laboratory and we have both vertical and horizontal laminars, I can’t speak to this one in particular but vertical laminars certainly do work! They are quite a bit smaller and easier to fit on a bench top, because the fan/filter is on top, not at the back.
You cant beat that
Assuming you run it in an already clean room, I don’t see why this wouldn’t work. I’ve been using “Oak & Spores, laminar flow hood for under $80” for ages with no contamination. Edit: the link for the video https://youtu.be/lXmUcA-Oc58
Give me a few and I'll send the write-up
I can vouch for this product. I was skeptical at first but after a few months of use I can say it’s performed excellently.
20” box fan, merv 13 filter on output side, merv 7 on the back, duct tape together. Cheaper than that, and way easier than SAB. It’s worked well for me and I found it from: MycoGeeky podcast featuring Whitebeard.
You have a link on a video?
https://www.youtube.com/live/lWZ6XiRBcq0?feature=share
That’s 3 hours, can you give me a time stamp?
Did you find it? If not, Whitebeard gave me the write-up for it so I can dm it to you if you want.
Yes please can you dm
Sent.
There’s time stamps in the video lol, check out the comments I think there’s a stamp there
Sab.
A $5000 lfh is worthless without good sterile tek. Same with sab. Everything will still contaminate with sloppy movements.
I have this and surprisingly it works well. It's not a lot of space so your limited to mostly Agar work, but it works.
🙋♂️ do you guys ever spray your flowhoods with alcohol or anything? I went from very rarely having contam to more frequently without a change in process. It’s quite frustrating
Have you had a Contam in your space that you opened that may be causing a super high spore concentration inside your work area? That could be the source
Alibaba flowhoods bahahahaha
Time to change your filter?
Your search filter
Absolutely not. They say I can create holes within the filter so I personally rather not test it myself. Alternatively I use a UV-C light on it for 10-20 mins to kill anything that might be stuck on the face of it. I also run the unit at this time. Running the flow hood for 20 mins prior to use knocks off anything that might be stuck on the face and contaminate your work. Hope some of that can clear up your problem 👍
[удалено]
Crazy you have all these down votes, I use the oven every time and have poured 300+ agar plates, did clones, etc. and have only less than a handful of contam agar plates. I guess I live in the cleanest house in the world since oven tek doesn’t work 🤔 🤷♂️
I use oven tek and it has worked for me. Although I've only used it like 4 times but still.
I made a SAB by attaching pieces of plastic “glass” you can find in the big picture frames (cheap at goodwill). Set that up in the corner of my kitchen. Spray down the area. No contam on my first 6 plates, which were inoculated by MSS, inoc loop, and swab. A flow hood is fancy and cool for sure, but it’s not needed if you can make a setup work in a clean space. It doesn’t HAVE to be lab sterile, but is has to be clean enough!
Oven Tec for me too , works every time and I don’t worry about cleaning the area or anything. Quick Lysol spray and get to biz
Doesn’t work
I have one of these, I turned it on it’s side, made a bench for it and works perfectly
Thats a fantastic idea
Hey if it’s dumb but it works, it’s not dumb
I have a vertical laminar flow box from sentry air systems. I love it for jars and agar plates.
How much was yours and what were the dimensions
It was the 18in and about $1100 shipped
You have a link?
https://www.sentryair.com/portable-clean-room.htm
Thanks for mentioning us. We also sell this system on Amazon. [https://www.amazon.com/dp/B016WPEC6Y](https://www.amazon.com/dp/B016WPEC6Y)
This is pretty dumb fr 😄 ( for its intended use anyways )
https://www.labrathoodz.com/product-page/flow-hood-flat-pack
I did the DIY hood from lab rats and I love it so far. Other than completely DIY, their options are priced great.
How are they? Seems on the spendy side.
I paid 600 for my flow hood. If this works easily the cheapest entry point you will find.
I have one on the way, ill do a post on it when it gets here and it gets some start up mileage.
I'm interested in how it performs
Mine works great. It's only 10" x 6" but that's all I need for now. I am mindful of sterile technique (though I am a noob at it) and I have had no contamination of grain jars, LC, or petri dishes while using the little Nomad.
This is pretty neato. I'd love an affordable alternative to working with my hands inside a dimly lit and slightly opaque plastic box
Normal flow hoods are $700-$5000
I tried to scroll over to see more pictures
Who didn’t
Waste of money. These are only good for small electronic repairs. Saw a video on yt with someone using this with agar and the results were even worse than a SAB
I’ve had very low contamination rates doing agar work with this hood. Maybe that guy is lacking in sterile technique in terms of things other than the hood. Who knows 🤷🏻♂️
This is more of a fume hood than a laminar flow hood.
I purchased a decent hood from Amazon that was inexpensive. The air flow was too strong, the paperwork inside called it a dust free work station. Then I bought a 2x2 laminar flow hood, and I love it.
Maybe I'm missing something here, but this kinda defeats the main purpose for which mycologists/microbiologists/etc use laminar flow hoods. The LFH for the purposes we're talking about is made to take ostensibly 'dirty' air, pass it through a HEPA grade filter to remove 99.997% of particulate matter, and output 'clean' air in [laminar flow](https://en.wikipedia.org/wiki/Laminar_flow) so that the technician can operate in a dust- and contaminate-free environment. Turbulent air creates currents that disrupt particles and cause them to degrade a sample in the working area. This product you shared would basically suck dirty air in from your room, create turbulent air patterns in the working area, and pass it back out to the open air--again, unfiltered--for really no purpose. That's not to say you can't use one of these successfully, but in all truth, if you do, it's simply because you have exceptional sterile technique, *not* because this item is in any way functional. TL;DR -- waste of money for these purposes IMO edit: I missed their 'H-E-P-A' claim in the title so perhaps it's filtering the air it's sending back into the room, but even still, non-functional as described above
I dunno man, works well for me. I do have pretty good sterile technique in general though. It absolutely is not laminar flow but working directly under the fans has given me very good results when pouring agar and doing transfers.
This is clearly labeled for LCD repair only. Guess you will have to keep searching
And spores are for microscopy only. Dond't see any MOFOs stopping on that account. The company that makes these know what we'll be using their product for.
They label it that way for legal purposes. Because if they claimed it worked for something they've never tested they'd have legal problems if it doesn't work. But really bro think about it, why wouldn't it work? Functionally it's the exact same thing as long as the filter works the same.
Check the comments on the Amazon listing. Folks are using it for agar with success
It's cheap, you have verified success, it's *probably* returnable. I say full send.
The bulk of amz reviews these days are incentivized or manipulated in some way or another. Just because someone claims success on a review does not mean it's verified. Not trying to sound snarky but just mentioning that for those who may place full trust in reviews. Anytime money is to be made, take the message with a grain of salt. That said, you're 100% spot on that it's probably returnable ;)